[Seminars] FW: [physcolloqadmin] Physics Colloquium Thursday June 10, 2021 at 3:30pm CDT with Sung Joon Kim

[Ramona Echols]

[cidimage001.png at 01D73CEF.65EEAD30]

Characterization of oritavancin mode of action and Staphylococcus aureus peptidoglycan structure by solid-state NMR

 [A person smiling for the camera  Description automatically generated with low confidence]

Sung Joon Kim

Howard University

3:30pm CDT, Thursday, June 10, 2021

ZOOM Link:
<https://urldefense.com/v3/__https:/uchicagogroup.zoom.us/j/96367028599?pwd=VU9nOHZ1YW1hUnZGVXNPTVBhOWdkdz09__;!!BpyFHLRN4TMTrA!tt_kV2_FH9A631m17zfQHAyPu9DvDkxCiPRRA50o-8NkWhd6H2b5zfkWBTtv0kSZXw$>https://uchicagogroup.zoom.us/j/96367028599?pwd=VU9nOHZ1YW1hUnZGVXNPTVBhOWdkdz09<https://urldefense.com/v3/__https:/uchicagogroup.zoom.us/j/96367028599?pwd=VU9nOHZ1YW1hUnZGVXNPTVBhOWdkdz09__;!!BpyFHLRN4TMTrA!t4lZ1DWk7MM1JPXqBb126PvdjheDoK4dTZ1AtVcw39a3I6D_xaJD7I7DI0un9oeEBQ$>
Solid-state NMR was used to characterize the mode of action of oritavancin, a second-generation glycopeptide antibiotic reserved for the treatment of serious infections by multi-drug resistant Gram-positive pathogens.  Oritavancin inhibits bacterial cell wall biosynthesis by targeting the nascent peptidoglycan.  Rotational-echo double resonance (REDOR) NMR was used to determine the internuclear distances from the 19F of oritavancin to the specific 31P, 13C, and 15N labels that are incorporated into the cell walls of Staphylococcus aureus.  13C{15N} and 15N{13C} REDOR NMR confirmed that the potent bactericidal activity of oritavancin is due to dual inhibition of transglycosylation and transpeptidation steps of peptidoglycan biosynthesis by targeting the lipid transporter C55.  Since C55 is a shared transporter required for both peptidoglycan and wall teichoic acid (WTA) biosyntheses, we found that S. aureus treated with a sub-inhibitory concentration of oritavancin rapidly inhibited WTA biosynthesis, but without detectable changes to the peptidoglycan cross-link or stem-link densities.  The result is consistent with oritavancin targeting WTA prior to the peptidoglycan biosynthesis in S. aureus.


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Tiffany Kurns, Undergraduate Affairs Administrator
Department of Physics, Instructional Services Office
5720 S. Ellis Avenue, KPTC 205
Chicago, IL. 60637
Phone: 773-702-7019
tkurns at uchicago.edu<mailto:tkurns at uchicago.edu>



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